Lesões intestinais em suínos abatidos no Rio Grande do Sul / Intestinal lesions in pigs slaughtered in Rio Grande do Sul, Brazil

O Rio Grande do Sul se destaca na suinocultura nacional pelos altos índices de produção, baseada em sistema de criação intensivo, fato que propicia a ocorrência de doenças entéricas. Este estudo foi realizado para diagnosticar e determinar a frequência das causas de condenação de intestinos na linha de abate. As amostras de intestino e linfonodos mesentéricos foram colhidas para análise histológica e imuno-histoquímica, de um frigorífico de suínos localizado na Região do Vale do Taquari, no Rio Grande do Sul (RS), que conta com Serviço de Inspeção Federal (SIF). A colheita ocorreu durante 18 dias nos meses de verão (janeiro e fevereiro) e 19 dias nos meses de inverno (junho, julho e agosto) de 2016. Os suínos eram provenientes de 55 municípios diferentes, que fazem parte de sete microrregiões do RS (microrregião de Passo Fundo, Soledade, Guaporé, Santa Cruz do Sul, Lajeado-Estrela, Montenegro e São Jerônimo). Dos 2.523 intestinos condenados no verão e 2.747 no inverno, 216 e 199 foram avaliados, respectivamente. Os três principais diagnósticos foram serosite crônica (43,1%), ascaridiose (21,4%) e enteropatia proliferativa suína (20%), seguidos por casos de tampão caseoso em válvula ileocecal (5,0%), abscesso intestinal (1,0%), pneumatose (1,0%), circovirose (0,7%), esofagostomíase (0,7%), salmonelose (0,7%) e um caso de intussuscepção (0,2%). A serosite crônica foi diagnosticada em 82/216 (38%) no verão e 97/199 (48,7%) no inverno, ascaridiose teve maior ocorrência no verão com 64/216 (29,6%) comparado com o inverno em que observou-se 25/199 (12,6%). A enteropatia proliferativa suína foi diagnosticada em 37/216 (17,1%) casos no verão e 46/199 (23,1%) no inverno.(AU)

Rio Grande do Sul stands out in the national swine industry due to high production rates, based on an intensive breeding system, which leads to the occurrence of enteric diseases. This study was performed to diagnose and determine the frequency of causes of bowel condemnation in the slaughter line. The samples of intestine and mesenteric lymph nodes were collected for histological and immunohistochemical analysis of a pig slaughterhouse located in the Vale do Taquari area, in Rio Grande do Sul (RS), which has Federal Meat Inspection Service (SIF). The collection occurred during 18 days in the summer (January and February) and 19 days during winter (June, July and August) of 2016. The pigs were from 55 different counties from of seven microregions of RS (microregion of Passo Fundo, Soledade, Guaporé, Santa Cruz do Sul, Lajeado-Estrela, Montenegro and São Jerônimo). Out of 2,523 condemned intestines in the summer and 2,747 in the winter, 216 and 199 were evaluated, respectively. The three main diagnoses were chronic serositis (43.1%), ascaridiosis (21.4%), and porcine proliferative enteropathy (20%), followed by cases of plug caseous in ileocecal valve (5.0%), intestinal abscess (1.0%), pneumatosis (1.0%), circovirosis (0.7%), oesophagostomiasis (0.7%), salmonellosis (0.7%) and a case of intussusception (0.2%). Chronic serositis was diagnosed in 82/216 (38%) in summer and 97/199 (48.7%) in winter, ascaridiosis had a higher occurrence in the summer with 64/216 (29.6%) compared to the winter when it was observed 25/199 (12.6%). Porcine proliferative enteropathy was diagnosed in 37/216 (17.1%) in summer and 46/199 (23.1%) in winter.(AU)

Histological and immunohistochemical study on the possible protective effect of curcumin on intestinal ischemia / reperfusion induced lung injury in albino rats

Intestinal ischemia-reperfusion [I/R] injury may lead to severe local and remote tissue injury, especially acute lung injury. Curcumin has been shown to attenuate [I/R] injury in several organ systems. To evaluate the possible protective role of curcumin on acute lung injury induced by intestinal [I/R] in albino rat. Thirty male albino rats were divided equally into control group [Sham-operated], which underwent laparotomy without superior mesenteric artery occlusion. It was classified into two subgroups: subgroup a: which received no treatment, subgroup b: which received curcumin, I/R group where rats were exposed to superior mesenteric artery occlusion for 1 hour followed by 1 hour reperfusion. Third group was I/R and curcumin. Curcumin, was administered in a dose of [100 mg/kg] daily orally for 15 days before experiment. Rats were sacrificed at the end of reperfusion. Lung sections were stained with H and E and Cyclooxygenase -2 immunostain. A morphometric study and statistical analysis were performed. Intestinal I/R caused severe histological changes including massive infiltration of cells in thickened alveolar septa, around bronchioles and congested blood vessels. Curcumin led to obvious regression of induced histological changes by I/R. Significant increase in the mean thickness of inter alveolar septa and the mean area% of Cox-2 immunoexpression were recorded in [I/R] group compared to other groups. Curcumin administration proved a beneficial protective effect on I/R induced acute lung injury, and therefore has potential for clinical use

Anastomotic leakage after gastrointestinal surgery: risk factors presentation and outcome

Intestinal anastomosis is one of the most commonly performed surgical procedures, especially in the emergency setting and is also commonly performed in the elective setting when resections are carried out for benign or malignant lesions of the gastrointestinal tract. Anastomotic leak after gastrointestinal anastomosis is one of the important postoperative complication that leads to significant morbidity and adversely affects length of hospital stay. To define the risk factors, presentation and outcome of anastomotic leakage after gastrointestinal anastomosis. Prospective data collection from patients who underwent small or large bowel resection and anastomosis without fecal diversion in the surgical department in Al Zahraa University Hospital in the period between November 2010 and April 2014. Demographic details of the patients as well as preoperative, intraoperative and postoperative data were recorded. Leak found or not and on which postoperative day leak found. How it was identified [clinical or radiological] and how it was treated. Outcome of patients was recorded as mortality rate and postoperative hospital stay. There were 70 [63.64%] males and 40 [36.36%] female patients. Mean age was [44.23 +/- 15.78] years. Anastomotic leak was occurred in 17 [15.4%] patients group I, while there was no leak in 93 patients [84.6%] group II. The mean postoperative period for diagnosis of anastomotic leakage was 9 days range [5-16] days. Categorical variable found to be significantly affecting the outcome of anastomosis were age of the patients [P 2 unit [P

Multidetector CT Findings of Bowel Transection in Blunt Abdominal Trauma

OBJECTIVE: Though a number of CT findings of bowel and mesenteric injuries in blunt abdominal trauma are described in literature, no studies on the specific CT signs of a transected bowel have been published. In the present study we describe the incidence and new CT signs of bowel transection in blunt abdominal trauma. MATERIALS AND METHODS: We investigated the incidence of bowel transection in 513 patients admitted for blunt abdominal trauma who underwent multidetector CT (MDCT). The MDCT findings of 8 patients with a surgically proven complete bowel transection were assessed retrospectively. We report novel CT signs that are unique for transection, such as complete cutoff sign (transection of bowel loop), Janus sign (abnormal dual bowel wall enhancement, both increased and decreased), and fecal spillage. RESULTS: The incidence of bowel transection in blunt abdominal trauma was 1.56%. In eight cases of bowel transection, percentage of CT signs unique for bowel transection were as follows: complete cutoff in 8 (100%), Janus sign in 6 (100%, excluding duodenal injury), and fecal spillage in 2 (25%). The combination of complete cutoff and Janus sign were highly specific findings in patients with bowel transection. CONCLUSION: Complete cut off and Janus sign are the unique CT findings to help detect bowel transection in blunt abdominal trauma and recognition of these findings enables an accurate and prompt diagnosis for emergency laparotomy leading to reduced mortality and morbidity.

Complicações gastrintestinais em usuários de cocaína/crack: revisão da literatura / Gastrointestinal complications among users of cocaine / crack-a literature review

O consumo de cocaína/crack atinge todos os extratos sociais e grande parcela da população, principalmente os jovens. Esse abuso leva à ampla gama de complicações sistêmicas. No trato gastrintestinal, pode se expressar por manifestações como perfuração gastroduodenal aguda, colite isquêmica, infarto, isquemia intestinal e, raramente, hemorragia maciça. Seu mecanismo fisiopatológico parece ser o vasoespasmo ou vasoconstrição, que pode levar à isquemia, inclusive com necrose transmural. É importante a atenção e vigilância para o abuso de cocaína/crack ao deparar com paciente com dor abdominal inexplicável. (AU)

Cocaine/crack have being consumed by a large portion of the population especially by youth and reaching all social levels. This abuse leads to a wide range of systemic complications. In the gastrointestinal tract, the drug can lead to manifestations such as acute gastroduodenal perforation, ischemic colitis, infarction, intestinal ischemia and, rarely, massive hemorrhage. The most accepted pathophysiological mechanism is vasospasm or vasoconstriction which can lead to ischemia, including transmural necrosis. It is important that physicians to be aware and search recent history of abuse of crack / cocaine when faced with a patient with unexplained abdominal pain. (AU)

Fecaloma, oblito y oclusión intestinal: informe de un caso, comentarios y una propuesta / Fecaloma, foreign body and intestinal occlusion: a care report, comments and a proposal

Se presenta el caso de un paciente de 47 años con enorme giba lumbar derecha que apareció a los 18 años de edad. Fue operado hace 14 años de una formación quística con aumento de volumen de las extremidades y aparición, poco después, de un tumor de 2,5 cm. Diez días antes de su ingreso presentó infección urinaria, luego constipación, dificultad para expulsar gases, dolor y distensión abdominal, y se visualizaba la referida tumoración. En la radiografía se observaron múltiples fecalomas y oblito metálico. Fue operado y se constató la presencia de fecalomas, distensión de asas y el oblito cuya punta era la que se visualizaba y palpaba(AU)

This is a case presentation of a patient aged 47 with a huge right humpback appearing at 18 years old. He was operated on 14 years ago from a cystic formation increasing the volume of limbs and the appearance afterwards of a 2,5 cm tumor. Ten days before its admission showed a urinary infection, constipation, difficulty to release gases, pain and abdominal distention, visualizing such tumor. At radiography on noted many fecaloma and metallic foreign body. He was operated on verifying the presence of fecalomas, loop distention and foreign body whose tip was visualized and palpated(AU)

Renal subcapular saline injection during percutanoues renal cryoablation to prevent bowel injury.

INTRODUCTION: To evaluate the feasibility of percutaneous injection of saline in the renal subcapsular space to prevent bowel injury and histopathologic effects of bowel cryolesion during renal cryoablation in a porcine model. MATERIAL AND METHOD: Six pigs underwent percutaneous renal cryoablation with two freeze cycles in the lower pole of both kidneys. Six kidneys were injected with 10 ml saline into the renal subcapular space before cryoablation. The bowel was brought into contact with the edge of the ice ball with laparoscopic assistance during renal cryoablation, on the side with saline injection as well as on the control side. One of these animals was kept for survival follow-up and laparotomy for 7 days post cryoablation. The bowel cryolesion sites were observed and compared based on the presence or absence of renal subcapsular saline injection. RESULTS: The mean diameter of acute bowel injury with and without saline renal subcapsular injection was 7.25 +/- 1.26 and 14.5 +/- 0.58 mm, respectively. The influence of injecting a saline buffer was a significant decrease in the bowel cryolesion compared to controls (p = 0.0003). In addition, a pig kept for follow-up confirmed no bowel perforation after 7 days at a site that was cryolesioned on the side with renal subcapsular saline injection, but sustained bowel perforation in another segment lesioned by contact with a kidney without a saline injection. Gross and microscopic pathological examination was consistent with these interpretations. CONCLUSION: Preliminary results in a porcine model show that percutaneous renal subcapsular saline injection is a feasible and promising technique for preventing bowel complications of percutaneous image-guided renal cryoablation.

effects of N-acetylcysteine on intestinal ischemia/ reperfusion injury in rats

To evaluate the effects of N-acetylcysteine NAC on the injury of intestinal ischemia-reperfusion. Forty-eight Wistar-Albino rats were divided into 6 groups: as control, ischemia, ischemia-reperfusion, ischemia + N-acetylcysteine, ischemia-reperfusion + N-acetylcysteine IRN, and reperfusion + N-acetylcysteine RN. Histopathologic examination was performed to all groups. In the tissue and plasma, and erythrocyte samples, malondialdehyde, superoxide dismutase, glutathione, and nitric oxide NO levels were evaluated. The present study was carried out in Trakya and Istanbul University, Edirne, Turkey between December 2002 and July 2003. The most severe histopathological damage was seen in the intestinal ischemia-reperfusion group, and this damage was observed to be reduced by NAC administration. Lowest plasma malondialdehyde levels were observed in RN group. The tissue glutathione levels were found to be higher in RN group than those in IRN group. It was found that administration of NAC has important effects on the injury of intestinal ischemia, as well as, reperfusion in rats. N-acetylcysteine administration causes an improvement in the histopathologic findings of ischemia/reperfusion damages. The N-acetylcysteine treatment protects the antioxidant enzymes in the tissue, plasma, and the erythrocytes, which are crucially important in the intestinal schemia/reperfusion injury in rats

Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome / Lesões entéricas em suínos afetados por síndrome multissistêmica do definhamento dos suínos

Samples of mesenteric lymph nodes and intestines from 79 unthrifty 3- to 5-month-old postweaning pigs, confirmed as naturally affected with postweaning multisystemic wasting syndrome (PMWS), were studied. Pigs originated from 12 farms in southern Brazil and were selected on the basis of clinical signs and/or gross lesions suggestive of enteric disorder. Lymphohistiocytic infiltrates of varying intensity were associated with anti-porcine circovirus type 2 (anti-PCV2) immunostaining (IS) in samples of intestines and mesenteric lymph nodes from all pigs. Although most findings were similar to those described in PCV2-associated enteritis, anti-PCV2 IS in association with depletion of the goblet cell mucin stores (24 pigs), diffuse ileal villous atrophy and fusion (18 pigs), and dilatation of the lymphatic vessels (11 pigs) combined or not with lymphangitis were also observed. PCV2 antigen was immunohistochemically demonstrated in the cytoplasm and nuclei from intralesional epithelial cells, histiocytes, and endothelial-like cells in intestinal tissues. Together these findings imply an association with PCV2. The presence of co-infections by Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavirus, parvovirus, coronavirus and enteric calicivirus with PCV2 in the intestinal lesions was investigated.

Amostras de linfonodos mesentéricos e intestinos de 79 leitões desmamados refugos, entre 3 e 5 meses de idade e confirmados como naturalmente afetados pela síndrome multissistêmica do definhamento foram estudadas. Os suínos eram oriundos de 12 criações no sul do país e foram selecionados em função dos sinais clínicos e/ou lesões macroscópicas sugestivos de doença entérica. Infiltrados linfoistiocíticos de intensidades variáveis foram associados com marcação positiva anti-circovirus suíno tipo 2 (anti-PCV2) em amostras de intestinos e linfonodos mesentéricos de todos os 79 animais. Embora a maioria dos achados fossem semelhantes aos descritos em enterite associada com PCV2, marcação imuno-histoquímica anti-PCV2 foi associada com depleção de células caliciformes (24 suínos), atrofia e fusão de vilosidades do íleo (18 suínos) e dilatação de vasos linfáticos (11 suínos) combinada ou não com linfangite. Antígenos de PCV2 foram demonstrados por imuno-histoquímica no citoplasma e núcleo de células epiteliais intralesionais, histiócitos e células tipo endotelial em tecidos intestinais. Em conjunto, esses resultados sugerem que as lesões estavam associadas com PCV2. A presença de co-infecções por Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavírus, parvovírus, coronavírus e calicivírus entérico com PCV2 nas lesões intestinais foi investigada.

Effect of dietary arginine on the jejunal mucosa in indomethacin-induced intestinal injury: light and scanning electron microscopic study in male mice

The non-steroidal anti-inflammatory drug [NSAID] indomethacin causes, via its adverse effects, damage to the gastrointestinal mucosa of humans and experimental animals. The indomethacin-induced intestinal injury in mice jejunum is considered an experimental model of Crohn's disease, as one of the inflammatory bowel diseases [IBD's]. The semi-essential amino acid arginine, which is a precursor of nitric oxide, is proposed to promote gastrointestinal mucosal integrity. The present work aimed to study the possible protective role of dietary supplementation with arginine in ameliorating indomethacin-induced mucosal injury of mice jejunum. The present study was carried out on forty adult male mice which were divided into 4 equal groups; group I [negative control group] which received no treatment, group II [positive control]; mice received dietary L-arginine alone in a daily dose of 300 mg/Kg, group III [indomethacin group]; mice of this group received 2 consecutive subcutaneous injections of indomethacin in a dose of 7.5 mg/kg, 24 hours apart and group IV [arginine group]; in which arginine supplementation was provided 2 days before the administration of indomethacin, maintained during the administration and continued 3 days later till the end of the experiment. Mice of all groups were sacrificed by the end of the 7[th] day and specimens from the jejunum were dissected and processed for light and scanning electron microscopic examinations. Indomethacin-treated group exhibited jejunal mucosal injury. Light microscopic examination of this group, using H and E and toluidine blue- stained semithin sections showed distorted villi and sloughing of some of their apical parts with extrusion of many degenerated cells, intermingled with excess mucous. Some enterocytes appeared degenerated with loss of their regular arrangement, while the goblet cells appeared distended with excess mucous secretion. Increased cellular infiltration and edema of the villous core and in the lamina propria between the glands were noticed. Increased mucous secretion was also demonstrated by combined alcian blue-periodic acid Schiff reaction. Scanning electron microscope revealed alteration in the architecture of many villi which appeared short, blunt or with denuded surface and occasionally covered with membrane-like structure. The group of mice received arginine [group IV] revealed restoration of mucosal integrity in the form of regular villi with intact epithelial coverings including enterocytes and goblet cells. Some villi appeared shorter, while others showed partially denuded apical surface. Arginine evoked a remarkable cellular infiltration. Lymphocytes and macrophages were among the infiltrating cells and their roles were suggested to be vital in the healing process. Dietary L-arginine provided satisfactory protection against indomethacin-induced mucosal injury in mice, most probably via its role as a nitric oxide donor. So supplementation with dietary arginine is recommended

Bowel injuries secondary to induced abortion: a dilemma

To study the pattern of bowel injuries incurred by induced abortion, and the morbidity and mortality associated with them. Prospective descriptive study from December 2002 to December 2005. Surgical Unit three Gynaecology and Obstertrics Unit two, Civil Hospital, Karachi. All patients with bowel injuries due to induced abortion. Detailed data of all the patients was collected and analyzed. A total of 22 patients, mostly young with an average age of 26.86 years, presented with bowel injuries following induced abortion. Severe hemorrhage occurred in 8[36.4%] patients while 11[50%] had ileal perforation; 9[40.9%] underwent primary repair and 2[9.1%] ileostomy formation. Two [9.1%] patients with jejunal perforation had primary repair, whereas two with both jejuna and ileal perforations underwent resections with anastomosis in one and ileostomy in another. Seven [31.8%] with large gut involvement had colostomy formation. Septicemia and wound infection occurred in 7[31.8%] patients each, faecal fistula and abdominal wound dehiscence in 3[13.6%], and pelvic abscess in 1[4.6%] patients. The total mortality in this series was 6[27.3%] patients. Iatrogenic injuries during induced abortion, most commonly caused by quacks, can be minimized substantially if the procedure is performed by qualified medical personnel in proper health care facilities. There is a need for radical overhauling of the mind set in our society together with legislation

Manejo das lesões intestinais em cirurgia ginecológica / Intestinal injury management in gynecological surgery

As lesões iatrogênicas do intestino podem ocorrer em qualquer procedimento cirúrgico ginecológico, independente da via de acesso usada para realização da cirurgia. São pouco freqüentes, mas podem ter conseqüências drásticas para as pacientes quando não forem diagnosticadas ou reparadas da maneira adequada. Deve-se atentar para as pacientes que apresentam fatores de risco para complicações intestinais intra-operatórias, principalmente a possível ocorrência de aderências intestinais aos órgãos do sistema reprodutor feminino, e tomar medidas preventivas em relação às mesmas. O diagnóstico precoce destas iatrogenias propicia normalmente reparo simples das lesões, não sendo necessário colostomia, o que diminui a morbimortalidade destas pacientes.

Laparoscopy in gynaecology; complications and avoidance

The purpose of this paper is to carry out a systematic review of the literature regarding the complications of laparoscopic surgery in gynaecology; their incidence, causes and ways of prevention. The minimally invasive surgery has established wide acceptance by the patients because of its well recognized advantages. However, the associated complications of the surgical procedures are not perceived at the same level. The possibility of occurrence of complications with the new techniques and the new technologies are well recognized. The role of the surgeon in laparoscopic surgery is explored regarding the safety of the procedure

Lesões intestinais decorrentes de isquemia e reperfusão: fisiopatologia e modelos experimentais / Reperfusion injury intestinal ischemia: pathophysiology and experimental models

A isquemia intestinal é uma emergência pouco frequente em cirurgia vascular. As principais causas dessa doença são as embolias e as tromboses arteriais. A isquemia, por si só, é bastante lesiva, mas a reperfusão do tecido isquêmico pode levar a uma série de complicações, que podem aumentar as lesões teciduais e, associado às complicações sistêmicas, colocar em risco a vida do paciente. As lesões decorrentes da isquemia e reperfusão em tecido intestinal foram demonstradas em estudos clínicos e experimentais, nos quais estudou-se também a fisiopatologia e os tratamentos adequados. Esses estudos foram feitos em grande variedade de modelos experimentais, e, consequentemente, os resultados encontrados foram bastante contraditórios...

Safety, pitfalls and early complications of laparoscopic ventral hernia repair

Ventral hernia repair, particularly with large defects are plagued by high recurrence and frequent wound complications. Laparoscopic ventral hernia repair [LVHR] potentially offers a tension-free technique with less morbidity and fewer recurrences. This study addresses safety, pitfalls and early complications associated with LVHR. Patients underwent LVHR in the period between January 2002 and May 2003 Preoperative, Intraoperative and postoperative as well as follow up data is presented. Thirty-six patients [41 hernias] had attempted LVHR. Hernias were 27 incisional, 7 recurrent incisional, 6 primary and 1 iatrogenic. Mean operative time was 2 hours [range 1-4]. Mean hospital stay was 2.4 days [range 1-10]. Two cases underwent concomitant additional procedures. Two cases [5.5%] were converted to open procedure; 2 others needed open re-exploration. Complications included 3 cases [8.3%] of inadvertent bowel injury. Twenty-two cases [68.7%] had wound complications; none needed operative intervention. Pain was the most common reason delaying patient discharge. Mean follow-up was 22 months [range /2-28 months] with 2 [7.14%] recurrences. LVHR is safe however, it may be associated with significant morbidity, in part preventable by proper patient selection, meticulous technique and liberal conversion to open technique. Benefits for LVHR include proper repair of all defects, without long incision or extensive abdominal wall dissection. Although wound morbidity seems common, most are minor and resolve spontaneously

Evaluation of extra mucosal single layer intestinal anastomosis technique

An insecure intestinal anastomosis is an unacceptable iatrogenic hazard. The breakdown of suture line or anastomosis may result into hemorrhage, leakage, Stenosis, diverticular formation and ultimately faecal fistula with serous faecal and septic complications. The sound haeling of process of anastomosis depends mainly on anastomosis technique, which is the most important determinant. Therefore the main objective of this study was to assess/evaluate anastomotic technique. This cohort study with 50 cases necessitating intestinal anastomosis was designed at CMH Pano Aqil from January 2001 to August 2002. This comprised of 40 [80%] males, 10 [20%] females, which were mainly young adults. Patients with gunshot, sharp and blunt object trauma to the abdomen/gun were 35[70%] alongwith 15 [30%] patients in whom intestinal perforation was non traumatic in origin. Only one case showed minor anastomotic leak, which settled without surgical intervention. It is concluded that extramucosal single layer intestinal anastomosis is simple, safe, easy and logical technique

Lesão intestinal após isquemia-reperfusão: estudo comparativo usando sal tetrazólico (MTT) e histologia / Intestinal injury after ischemia-reperfusion: comparative study using tetrazolium salt (MTT) and histology

Vários métodos são utilizados para avaliar e estimar as lesões intestinais de isquemia e reperfusão (IR). Assim, o objetivo do presente trabalho é realizar o estudo comparativo dos aspectos colorimétrico e histológico da lesão intestinal após IR. Para tal, foram utilizados 30 ratos Wistar, machos, pesando entre 310 a 410g, distribuídos em 3 grupos: Grupo Controle (GC), Grupo Isquemia e Reperfusão-1 (GIR-1) e Grupo Isquemia e Reperfusão-3 (GIR-3), com 10 animais cada. Nos grupos GIR-1 e GIR-3 foi realizada isquemia intestinal, por meio de falsa ligadura da artéria mesentérica anterior, durante 30 minutos e após esta a perfusão sangüínea foi restaurada. Estes animais foram submetidos a eutanásia após 1 e 3 dias de reperfusão, respectivamente, sendo colhido material para realização dos estudos colorimétrico, usando o Methyl Thiazolyl Blue (MTT) e histológico pela hematoxilina e eosina. Os resultados obtidos demonstraram uma menor proporção de células viáveis e um maior grau de lesão da túnica mucosa nos animais do grupo GIR-3 em relação ao controle (p<0,05). Desta forma os autores concluem que o estudo colorimétrico, usando o MTT, mostrou-se tão eficaz e confiável quanto o estudo histológico na avaliação das repercussões intestinais produzidas pela IR deste órgão.